using System; using System.IO; using System.Threading; using AutoFocusTool.Serial; using AutoFocusTool.Imaging; using SerialCamera = AutoFocusTool.Camera.Camera; namespace AutoFocusTool { /// /// 只读诊断工具:逐个 well 移动到其 EEPROM 水平位置 → 抓帧 → 跑 WellDetector → 存图。 /// 用于验证“每个 well 移动过去后相机实际看到的是不是对应的孔、偏差多少”。 /// 不做对焦/不改标定,只在固定 Z(传入或默认)和固定曝光下拍一张。 /// /// 用法:WellSpacing.exe [COM口=COM11] [相机index=2] [曝光=80] [Z=93000] /// 存图目录:calib_result\well_check_yyyyMMdd_HHmmss\ /// internal class WellSpacing { [STAThread] static int Main(string[] args) { Console.OutputEncoding = System.Text.Encoding.UTF8; if (args.Length > 0 && args[0] == "zcurve") return ZCurve(args); string port = args.Length > 0 ? args[0] : "COM11"; int camIdx = args.Length > 1 && int.TryParse(args[1], out int ci) ? ci : 2; int exposure = args.Length > 2 && int.TryParse(args[2], out int ex) ? ex : 80; int zPos = args.Length > 3 && int.TryParse(args[3], out int z) ? z : 93000; int camW = 2592, camH = 1944; void L(string m) => Console.WriteLine($"{DateTime.Now:HH:mm:ss} {m}"); string stamp = DateTime.Now.ToString("yyyyMMdd_HHmmss"); string outDir = $@"C:\claudeFile\TL\AutoFocusTool\TestData\well_check_{stamp}"; Directory.CreateDirectory(outDir); L($"========== well 实拍检测 {port} cam#{camIdx} 曝光{exposure} Z{zPos} =========="); L($"存图目录: {outDir}"); var motor = new HouseMotor(port) { Log = null }; if (!motor.Open()) { L($"✗ 打开 {port} 失败(串口被占用?)"); return 1; } motor.MotorDelayMs = 1500; int sn = motor.ShakeHands(); L($"握手 houseSn = {sn}"); var cam = new SerialCamera(camIdx, camW, camH, exposure); int init = cam.Init(); if (init != 0) { L($"✗ 相机#{camIdx} 初始化失败 code={init}"); motor.Close(); return 2; } cam.SetOpMode(0); cam.SetExposure(exposure); motor.OpenLED(); Thread.Sleep(300); // 先到统一对焦 Z(所有 well 用同一 Z,只看水平是否对到不同孔) motor.VerticalMoveTo(zPos, 1500); try { for (int w = 1; w <= 16; w++) { int hpos = motor.ReadWellHorizontalPos(w); if (hpos < 0) { L($"well{w,2}: 读EEPROM位置失败,跳过"); continue; } motor.HorizontalMoveTo(hpos, 1500); Thread.Sleep(200); cam.GrabRgb(); // 丢弃第一帧 Thread.Sleep(100); cam.GrabRgb(); byte[] buf = cam.GetSourceBuffer(); var c = WellDetector.Detect(buf, camW, camH); string tag = c.Found ? $"圆心({c.Cx:F0},{c.Cy:F0}) R={c.Radius:F0} 偏移X={c.OffsetXPct:F1}% Y={c.OffsetYPct:F1}% 完整={c.Complete}" : $"未检出圆[{c.RejectReason}]"; L($"well{w,2}: EEPROM水平={hpos,7} {tag}"); string detTag = c.Found ? (c.Complete ? "OK" : "partial") : "NOCIRCLE"; string path = Path.Combine(outDir, $"well{w:D2}_hp{hpos}_{detTag}.bmp"); ImageConverter.SaveBmp(buf, camW, camH, path); } } finally { motor.CloseLED(); cam.Dispose(); motor.Close(); } L($"========== 完成,图片在: {outDir} =========="); return 0; } /// /// Z清晰度曲线扫描:移到指定well的EEPROM水平位置,从zLo到zHi逐层抓帧算清晰度, /// 同时记录灰度均值(验证亮度归一化是否带偏),每层存图。 /// 用法:WellSpacing.exe zcurve <相机idx> [zLo=20000] [zHi=120000] [zStep=2000] [曝光=60] /// static int ZCurve(string[] a) { string port = a.Length > 1 ? a[1] : "COM11"; int camIdx = a.Length > 2 && int.TryParse(a[2], out int ci) ? ci : 2; int well = a.Length > 3 && int.TryParse(a[3], out int wv) ? wv : 1; int zLo = a.Length > 4 && int.TryParse(a[4], out int v4) ? v4 : 20000; int zHi = a.Length > 5 && int.TryParse(a[5], out int v5) ? v5 : 120000; int zStep = a.Length > 6 && int.TryParse(a[6], out int v6) ? v6 : 2000; int exposure = a.Length > 7 && int.TryParse(a[7], out int v7) ? v7 : 60; int camW = 2592, camH = 1944; void L(string m) => Console.WriteLine($"{DateTime.Now:HH:mm:ss} {m}"); string stamp = DateTime.Now.ToString("yyyyMMdd_HHmmss"); string outDir = $@"C:\claudeFile\TL\AutoFocusTool\TestData\zcurve_w{well}_{stamp}"; Directory.CreateDirectory(outDir); L($"==== Z清晰度曲线 well{well} {port} cam#{camIdx} Z[{zLo},{zHi}] step{zStep} 曝光{exposure} ===="); L($"存图: {outDir}"); var motor = new HouseMotor(port) { Log = null }; if (!motor.Open()) { L($"✗ 打开 {port} 失败"); return 1; } motor.MotorDelayMs = 1500; int sn = motor.ShakeHands(); L($"握手 houseSn={sn}"); int hpos = motor.ReadWellHorizontalPos(well); if (hpos < 0) { L("✗ 读well水平位置失败"); motor.Close(); return 1; } var cam = new SerialCamera(camIdx, camW, camH, exposure); if (cam.Init() != 0) { L("✗ 相机初始化失败"); motor.Close(); return 2; } cam.SetOpMode(0); cam.SetExposure(exposure); motor.OpenLED(); Thread.Sleep(300); motor.HorizontalMoveTo(hpos, 1500); L($"已移到 well{well} 水平={hpos}"); double bestScore = -1; int bestZ = zLo; try { for (int z = zLo; z <= zHi; z += zStep) { motor.VerticalMoveTo(z, 1500); Thread.Sleep(150); cam.GrabRgb(); Thread.Sleep(80); cam.GrabRgb(); byte[] buf = cam.GetSourceBuffer(); int roiW = (int)(camW * 0.4), roiH = (int)(camH * 0.4); var roi = new System.Drawing.Rectangle((camW - roiW) / 2, (camH - roiH) / 2, roiW, roiH); double score = Sharpness.Compute(buf, camW, camH, roi); double mean = GrayMean(buf, camW, camH, roi); L($"z={z,6} 归一化分={score:F5} 亮度均值={mean:F1}"); if (score > bestScore) { bestScore = score; bestZ = z; } ImageConverter.SaveBmp(buf, camW, camH, Path.Combine(outDir, $"z{z}_s{score:F4}.bmp")); } } finally { motor.CloseLED(); cam.Dispose(); motor.Close(); } L($"==== 峰值 z={bestZ} 分={bestScore:F5} 图在 {outDir} ===="); return 0; } /// 中央ROI灰度均值(BGR→灰度,行优先)。 static double GrayMean(byte[] bgr, int W, int H, System.Drawing.Rectangle roi) { long sum = 0; int n = 0; int stride = W * 3; for (int y = roi.Y; y < roi.Y + roi.Height && y < H; y++) for (int x = roi.X; x < roi.X + roi.Width && x < W; x++) { int p = y * stride + x * 3; sum += (bgr[p] * 29 + bgr[p + 1] * 150 + bgr[p + 2] * 77) >> 8; n++; } return n > 0 ? (double)sum / n : 0; } } }